Diversity of Both the Cultivable Protease-Producing Bacteria and Their Extracellular Proteases in the Sediments of the South China Sea |
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Authors: | Ming-Yang Zhou Xiu-Lan Chen Hui-Lin Zhao Hong-Yue Dang Xi-Wu Luan Xi-Ying Zhang Hai-Lun He Bai-Cheng Zhou Yu-Zhong Zhang |
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Affiliation: | (1) State Key Lab of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan, 250100, People’s Republic of China;(2) Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, 266555, China;(3) Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; |
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Abstract: | Protease-producing bacteria are known to play an important role in degrading sedimentary particular organic nitrogen, and yet, their diversity and extracellular proteases remain largely unknown. In this paper, the diversity of the cultivable protease-producing bacteria and their extracellular proteases in the sediments of the South China Sea was investigated. The richness of the cultivable protease-producing bacteria reached 106 cells/g in all sediment samples. Analysis of the 16S rRNA gene sequences revealed that the predominant cultivated protease-producing bacteria are Gammaproteobacteria affiliated with the genera Pseudoalteromonas, Alteromonas, Marinobacter, Idiomarina, Halomonas, Vibrio, Shewanella, Pseudomonas, and Rheinheimera, with Alteromonas (34.6%) and Pseudoalteromonas (28.2%) as the predominant groups. Inhibitor analysis showed that nearly all the extracellular proteases from the bacteria are serine proteases or metalloproteases. Moreover, these proteases have different hydrolytic ability to different proteins, reflecting they may belong to different kinds of serine proteases or metalloproteases. To our knowledge, this study represents the first report of the diversity of bacterial proteases in deep-sea sediments. |
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