Lack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli |
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| Authors: | Murphy Mark Schmid Nuoffer Stefanie Bickle Thomas A |
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| Institution: | Division of Molecular Microbiology, Biozentrum of the University of Basel, CH-4056 Basel, Switzerland. |
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| Abstract: | Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP. |
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