Metabolism of [14C]GA20 in a Cell-Free System from Developing Seeds of Phaseolus vulgaris L. |
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Authors: | Kwak, Sang-Soo Kamiya, Yuji Takahashi, Masahiro Sakurai, Akira Takahashi, Nobutaka |
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Affiliation: | The Institute of Physical and Chemical Research Wako-shi, Saitama 351-01, Japan |
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Abstract: | The metabolism of [14C]GA20 during seed maturation of Phaseolusvulgaris was studied using cell-free systems from embryos rangingin age from 10 DAF (day after flowering) to 24 DAF. Enzyme preparationsfrom very immature seeds actively converted GA20 to GA1, GA5and GA6. The ratio of incubation products suggested the biosyntheticpathway of GA20GA5GA6. Fluctuation in the levelsof endogenous C19-GAs, namely GA1, GA4, GA5, GA6, GA8, GA9 andGA20 was analyzed by GC-SIM using internal standards to compareenzyme activity with the levels of endogenous GAs. AlthoughGA1, GA4 and GA6 showed maximum levels on 20 DAF, enzyme activitydecreased during seed maturation and showed weak activity on20 DAF. 1Graduate student of the University of Tokyo, Department ofAgricultural Chemistry, Bunkyo-ku, Tokyo 113, Japan.3Present address: Pesticides Research Laboratory, TakarazukaResearch Center, Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo655, Japan. (Received December 17, 1987; Accepted March 30, 1988) |
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