Packed-bed reactor of immobilized bacterial bioluminescence enzymes: A potential high-sensitivity detector for automated analyzers |
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| Authors: | Kalevi Kurkijärvi Raimo Raunio Timo Korpela |
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| Institution: | Department of Biochemistry, University of Turku, SF-20500 Turku 50 Finland |
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| Abstract: | Commercial bacterial bioluminescence enzymes were immobilized on agarose with 85% recovery of coupled activity. A light-emitting continuous-flow column for NADH monitoring at picomole levels was developed. The present enzyme reactor exhibited good operational stability in continuous use, which makes the system applicable to a sensitive detector for automated analyzers. |
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