A unique activity assay for carboxypeptidase A in human serum |
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| Authors: | Lynn M Peterson Barton Holmquist JL Bethune |
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| Institution: | 1. Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Brigham and Women''s Hospital, Boston, Massachusetts 02115 USA;2. Department of Surgery, Harvard Medical School, Brigham and Women''s Hospital, Boston, Massachusetts 02115 USA;3. Department of Radiology, Harvard Medical School, Brigham and Women''s Hospital, Boston, Massachusetts 02115 USA |
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| Abstract: | Measurement of carboxypeptidase A, one of the pancreatic proteolytic enzymes, in human serum is made possible by a combination of affinity chromatography to isolate and concentrate the enzyme followed by monitoring activity spectrophotometrically with a high-turnover peptide substrate. Concentrations of enzyme in the nanogram-per-milliliter range can be determined with high precision and reliability. Initial clinical application of this method demonstrates no detectable activity in serum from normal individuals, but the enzyme is present in the sera of individuals with pancreatitis. |
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| Keywords: | To whom correspondence should be addressed |
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