Avidin as a precipitant for biotin-labeled antibody in a radioimmunoassay for carcinoembryonic antigen |
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| Authors: | Brian R Clark Charles W Todd |
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| Institution: | Division of Immunology, City of Hope Research Institute, Duarte, California 91010 USA |
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| Abstract: | Avidin is used in conjunction with polyethylene glycol to coprecipitate biotin-labeled carcinoembryonic antigen (CEA) antibody and the CEA-antibody complex together with biotin-labeled normal serum proteins. The procedure provides a reliable separation technique for a CEA radioimmunoassay. The technique is broadly applicable to assays employing immune precipitation. |
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