Measurement of endogenous leucine enkephalin in canine caudate nuclei and hypothalami with high-performance liquid chromatography and field-desorption mass spectrometry |
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| Authors: | Shigeto Yamada Dominic M Desiderio |
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| Institution: | 1. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Center for Health Sciences, 800 Madison Avenue, Memphis, Tennessee 38163 USA;2. Department of Neurology, University of Tennessee Center for Health Sciences, 800 Madison Avenue, Memphis, Tennessee 38163 USA |
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| Abstract: | For the first time, endogenous amounts of Leu-enkephalin are measured in brain tissue with a technique preserving integrity of the entire molecular structure of the neuropeptide. Field-desorption mass spectrometry enables measurement of picomole amounts of endogenous, chemically underivatized Leu-enkephalin in canine caudate nuclei and hypothalami. The optimal sensitivity and resolution of high-performance liquid chromatography is coupled with maximal molecular specificity of field-desorption mass spectrometry to measure enkephalins in caudate nuclei and hypothalami from dog brains. This novel combination of two recent instrumental methodologies provides a firm molecular basis for calibrating the radioimmunoassay measurement of endogenous levels of biologically active brain neuropeptides. |
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| Keywords: | To whom all correspondence and reprint requests should be addressed |
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