Radiation inactivation of enzymes at low dose rates: Identical molecular weights of rat liver cytosolic and lysosomal neuraminidases |
| |
| Authors: | Guy Beauregard Michel Potier |
| |
| Affiliation: | Section de Génétique Médicale, Hôpital Sainte-Justine, Université de Montréal, Montréal, Québec H3T 1C5, Canada |
| |
| Abstract: | A low-dose-rate gamma source (60Co) was calibrated with enzymes of known radiation inactivation behaviors and used for molecular weight determination of rat liver neuraminidase (EC 3.2.1.18). The method allows direct comparison of radiation inactivation of standard and unknown enzymes under identical experimental conditions. The membrane-bound lysosomal neuraminidase had the same molecular weight (Mr = 56,000 ± 8500) as the soluble cytosolic neuraminidase (Mr = 56,000 ± 7000) although they differ in their kinetic properties and substrate specificity. |
| |
| Keywords: | Author to whom correspondence should be addressed at: Section de Génétique Médicale Hôpital Sainte-Justine 3175 Chemin Sainte-Catherine Montréal Québec H3T 1C5 Canada. |
| 本文献已被 ScienceDirect 等数据库收录！ |
