Separation of basic,hydrophilic peptides by reversed-phase ion-pair chromatography: II. Analytical applications with particular reference to phosphoserine peptides |
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| Authors: | Bengt Fransson Ulf Ragnarsson Örjan Zetterqvist |
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| Institution: | 1. Institute of Biochemistry, Biomedical Center, University of Uppsala, Box 576, S-751 23 Uppsala, Sweden;2. Institute of Medical and Physiological Chemistry, Biomedical Center, University of Uppsala, Box 575, S-751 23 Uppsala, Sweden |
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| Abstract: | Phosphoserine peptides, obtained by phosphorylation of synthetic precursors with cyclic AMP-dependent protein kinase, can be efficiently separated from the corresponding non-phosphorylated peptides and from each other by ion-pair high-performance liquid chromatography. All experiments were performed under isocratic conditions on a C18 column, using phosphate buffers with pH 3.2–4.5, n-hexane sulfonic acid as counter ion, and ethanol as organic modifier. |
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