A manual subtractive end-group determination of oligopeptides using fluorescamine or o-phthalaldehyde |
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Authors: | E Méndez |
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Institution: | Servicio de Endocrinología Experimental, Centro Ramón y Cajal, Carretera de Colmenar, Km 9100, Madrid 34, Spain |
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Abstract: | A new method for the end-group determination of peptides using the fluorogenic reagents fluorescamine or o-phthalaldehyde is described. The method is based on the property that the derivatives of the N-terminal amino group of peptides formed in solution after reaction with either reagent are resistant to acid hydrolysis. The N-terminal amino acid can be determined by simply comparing the amino acid analysis of the original peptide with the fluorescent derivative of the peptide. In general, the decrease of the N-terminal residue in the reacted peptides in 80–90% with fluorescamine and more than 90% with o-phthalaldehyde. Any N-terminal amino acid, with the exception of proline, can thus be determined. |
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