The determination of the specific activity of picomolar amounts of long-chain acylcarnitine esters |
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| Authors: | Richard Odessey Kenneth V Chace |
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| Institution: | Department of Physiology, University of Virginia Medical Center, Charlottesville, Virginia 22908 USA |
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| Abstract: | Measurement of the specific activity of cellular pools of long-chain acylcarnitines is complicated by interference of other labeled cellular lipids, especially phosphatidylcholine and sphingomyelin. To overcome these problems the lipid extract from rabbit aorta labeled with 1-14C]palmitate was treated with phospholipase C. Upon two-dimensional thin-layer chromatography, the long-chain acylcarnitines could be isolated in an area free of interfering radioactivity. Mobility of long-chain carnitines was inversely proportional to the fatty acid chain length. The amount of long-chain acylcarnitine was quantified from their carnitine content after alkaline hydrolysis using carnitine acetyltransferase. |
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| Keywords: | To whom all correspondence should be addressed at: Department of Physiology Louisiana State University Medical Center 1901 Perdido Street New Orleans Louisiana 70112 |
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