High-performance liquid chromatography of N,O-acylated sialic acids |
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Authors: | Ashok K Shukla Norbert Scholz Ernst H Reimerdes Roland Schauer |
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Institution: | 1. Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstrasse 40-60, D-2300 Kiel, West Germany;1. Institut für Chemie der Bundesanstalt für Milchforschung, Hermann-Weigmann-Strasse 1-27, D-2300 Kiel, West Germany |
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Abstract: | A rapid, isocratic high-performance liquid chromatographic method for the analysis of N-acetylneuraminic acid, N-glycolylneuraminic acid, and their O-acetylated derivatives is described. Separation of sialic acids and of other monosaccharides as sugar-borate complexes is achieved on an anion-exchange resin. The sialic acids elute as individual peaks after the other sugars tested. The method allows quantitative determination, for example, of amounts of N-acetylneuraminic acid as small as 10 nmol. On cation-exchange resin sialic acids cannot be differentiated, but can be separated from neutral and amino sugars, allowing the determination of as little as 3 nmol of total sialic acids. |
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Keywords: | To whom all correspondence should be addressed |
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