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Purification of pigeon liver malic enzyme by affinity chromatography |
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| Authors: | Jaw-Tang Chang Gu-Gang Chang |
| Institution: | Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China |
| Abstract: | A simple and rapid method for the purification of malic enzyme (EC 1.1.1.40) from pigeon liver is described. Malic enzyme in the crude tissue extract was partially purified by heat treatment, ammonium sulfate fractionation, and DEAE-cellulose chromatography. Final purification was achieved by affinity chromatography on immobilized N6-(6-aminohexyl)-adenosine 2′,5′-bisphosphate. Apparently homogeneous enzyme was obtained in 2 days with 54% yield. |
| Keywords: | To whom correspondence should be addressed This paper is derived from the thesis of J T C presented in partial fulfillment of the requirements for a M S degree |
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