High-performance liquid chromatography of proteins: Purification of the acidic isozyme of adenylosuccinate synthetase from rat liver |
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Authors: | Frederick B Rudolph Sandra W Clark |
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Institution: | Department of Biochemistry, Rice University, Houston, Texas 77001 USA |
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Abstract: | High-performance ion-exchange liquid chromatography was utilized for the purification of the acidic isozyme of adenylosuccinate synthetase from rat liver. Initial steps in the purification included ammonium sulfate fractionation and DEAE-cellulose and agarose-GTP affinity columns. The final steps were done on a SynChropak AX-300 anion-exchange support. The enzyme was purified 3000-fold with an overall yield of 10%. The enzyme preparation exhibited only one protein band on gel electrophoresis. |
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Keywords: | To whom all correspondence should be sent |
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