Direct determination of UDP-glucuronic acid in cell extracts by high-performance liquid chromatography |
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| Authors: | Tak Yee Aw Dean P. Jones |
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| Affiliation: | Department of Biochemistry, Emory University School of Medicine, Atlanta, Georgia 30322 USA |
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| Abstract: | A simple and sensitive method for the direct determination of UDP-glucuronic acid by high-performance liquid chromatography with simultaneous measurement of UDP-glucose was developed. Optimal resolution and separation of UDP-glucuronic acid was attained under isocratic conditions with the ion-pairing agent n-octylamine. Quantitation was sensitive down to 5 pmol for standards and for liver cell extracts. Because this method directly measures UDP-glucuronic acid, it can be used for quantitation in the presence of drugs that interfere with enzymatic methods. |
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| Keywords: | Present address: Department of Biochemistry University of California Berkeley Calif. 94720. Author to whom correspondence should be addressed. |
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