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Agarose-acrylamide gradient gel electrophoresis of proteins |
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| Authors: | Donna F Warren Michael A Naughton Louis M Fink |
| Institution: | 1. Department of Pathology, University of Colorado School of Medicine, 4200 East Ninth Avenue, Denver, Colorado 80262 USA;2. Department of Obstetrics and Gynecology, University of Colorado School of Medicine, 4200 East Ninth Avenue, Denver, Colorado 80262 USA |
| Abstract: | A new agarose-acrylamide gradient slab gel electrophoresis system is described. The preparation of this new gel has been facilitated by the use of agarose with a relatively low gelation temperature. Fractionation of marker proteins and crosslinked proteins from a subcellular cytoskeletal preparation on agarose-acrylamide gradient gels is compared to that found using other acrylamide gel electrophoresis systems. |
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