Assay for polyunsaturated fatty acids by argentation-thin-layer chromatography using commercial thin-layer plates |
| |
| Authors: | Mitsushi Inomata Fumimaro Takaku Yoshitaka Nagai Masaki Saito |
| |
| Affiliation: | 1. Department of Biochemistry, Tokyo Metropolitan Institute of Gerontology, Itabashi-ku, Tokyo 173, Japan;2. Department of Hematology, Jichi Medical School, Tochigi 329-04, Japan;3. Department of Biochemistry, School of Medicine, University of Tokyo, Tokyo, Japan |
| |
| Abstract: | A simple, rapid, and convenient procedure for silver nitrate impregnation of commercial precoated silica gel plates is described. Silica-gel plates (Silica gel 60, E. Merck) were sprayed with 40% silver nitrate in water, dried in air, and activated at 100°C for 30 min. Samples containing fatty acid methyl esters were applied as 0.5- to 1.0-cm streaks and developed with a solvent system of benzene:ethyl acetate (9:1, v/v). The plates were sprayed with 70% sulfuric acid saturated with potassium dichromate, and the spots were detected by careful heating at 120°C for 90 min. This procedure is useful for separation and isolation of various species of fatty acid methyl esters and for simple, rapid, and reproducible estimation of microgram quantities of materials by spectrodensitometry of the chromatogram. |
| |
| Keywords: | To whom all correspondence should be addressed. |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
