Enzyme-gas chromatography: Theoretical and experimental aspects |
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Authors: | S. Pulvin M. Sharan D. Thomas |
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Affiliation: | Laboratoire de Technologie Enzymatique, ERA 338 du CNRS, Université de Technologie de Compiègne, BP 233, 60206 Compiègne, France |
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Abstract: | A method based on the production of gas by enzymes was developed to determine the concentration of amino acids. The enzyme was immobilized by coreticulation on the external surface of a capillary silicone tube. The gas produced diffused through the silicone membrane to the lumen of the tube and was carried by a vector gas to a gas chromatograph. The amount of measured gas has been shown to be a function of the amino acid concentration. A model of the system that gave good agreement between experimental and calculated values was developed. |
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Keywords: | To whom all correspondence should be addressed. |
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