大豆子叶衰老的细胞学观察

以萌发后不同时期的大豆子叶为材料,通过普通光镜和荧光显微镜观察,分析了不同时期子叶细胞的结构变化及子叶细胞内蛋白质和淀粉含量的组织化学变化.结果表明,随着种子萌发时期的延长,子叶细胞内的蛋白质和淀粉含量逐渐减少,在子叶衰老过程中,细胞内蛋白质首先消耗殆尽,淀粉的消耗速度较蛋白质慢;大豆子叶细胞在萌发后第18天时出现典型的植物编程性死亡的形态学特征,子叶细胞内营养物质的消耗诱发子叶细胞发生细胞凋亡.

Cytological Observation on the Senescence of Soybean Cotyledon

The structure of cotyledons of soybean in different periods of germination was studied through light microscopy and fluorescent microscopy,and the change of protein and starch grains in the cotyledon cell were also observed.It indicated that proteins and starch grains in the cotyledon cell diminished little by little during the seed germination.Proteins in the cell would be completely used firstly,and starch grains would be consumed slowly.A typical morphological feature of PCD was identified on the 18~(th) day of the periods of the germination.The consumption of the nutrients in the cotyledons of soybean cells induced the cell apoptosis.