中国林蛙的分子系统关系

测定了 6种林蛙和用作外群的 2种侧褶蛙和 1种陆蛙的线粒体 12SrRNA基因序列 393bp。序列两两对位比较表明内外群间的位点替换率是 7 3%到 2 3 1% ,内群中物种间则为 0 0 %到 9 2 %。依据上述DNA序列 ,用距离法和最大简约法的系统发育分析表明 :①研究的 6种林蛙聚为一支 ,构成单系群 ,并有高的BPs值(90 %以上 )支持 ;② 6种林蛙可以分成 2个姐妹群 ,即中国林蛙、黑龙江林蛙和桓仁林蛙为一组 (BPs >94% ) ,峨眉林蛙、昭觉林蛙和镇海林蛙为一组 (BPs >5 0 % ) ;③昭觉林蛙与镇海林蛙有较近的亲缘关系 ;④中国林蛙的榆中种群与牡丹江种群间的遗传分化似乎达到了种级分化水平。

Phylogenetic Relationships of Chinese Brown Frogs Inferred from Mitochondrial DNA Sequence of 12S Ribosome RNA Gene

Aligned 393 base pairs of the mitochondrial 12S?rRNA gene from 6 species of Chinese brown frogs，and Fejervarya limnocharis，Pelophylax nigromaculata and P.plancyi selected as outgroup were sequenced.Pairwise comparisons showed that the site variability between ingroup and outgroup members ranged from 7.3% to 23.1%.The variability among ingroup members ranged from 0.0% to 9.2%.　　Phylogenetic analyses based on these DNA sequence data by the neighbor-joining and maximum parsimony methods showed：①These six species of Chinese brown frogs grouped together and formed a monophyletic clade (BPs value >90%).②The six species of ingroup were divided two sister groups，i.e.Rana chensinensis，R.amurensis，and R.huanrenensis clustered together (BPs value >94%)，and R.omeimontis，R.chaochiaoensis and R.zhenhaiensis formed another cluster (BPs value >50%).③R.chaochiaoensis was more closed to R.zhenhaiensis than R.omeimontis.④The Yuzhong population of R.chensinensis related to R.huanrenensis while the Mudanjiang population of R.chensinensis was close to R.amurensis.This indicates that the differentiation between the two populations of R.chensinensis is likely to be at species level.So R.kukunoris including the Yuzhong population of R.chensinensis and R.dybowskii including the Mudanjiang population of R.chensinensis may be revived as two valid species.