In vitro localization of the protein synthesis defect associated with experimental phenylketonuria |
| |
Authors: | Marc-André Elsliger Georges R. Thériault Didier Gauthier |
| |
Affiliation: | (1) Département de chimie et biochimie, Université de Moncton, E1A 3E9 Moncton, NB, Canada;(2) Present address: Department de génétique médicale, Hôpital Ste-Justine, H3T 1C5 Montréal, QC, Canada |
| |
Abstract: | We have used a cell-free system derived from hamster brain to investigate protein synthesis during experimental phenylketonuria. In such a system the elongation inhibitor emetine impeded translation in extracts derived from both treated and control animals. On the other hand the initiation inhibitor aurintricarboxylic acid showed no effects on protein synthesis activity of treated hamsters, although it was severely inhibiting in controls. This suggests that initiation is the altered step in brain protein synthesis failure consecutive to phenylketonuria.Abbreviations ATA aurintricarboxylic acid - HPA hyperphenylalaninaemia (hyperphenylalaninaemic) - PHE phenylalanine - PKU phenylketonuria (phenylketonuric) - PR polyribosome |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|