Deleterious effect of minimal enzymatic treatments on the development of isolated maize embryo sacs in culture |
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Authors: | Nathalie Leduc Elisabeth Matthys-Rochon Christian Dumas |
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Institution: | (1) Laboratoire de Reconnaissance Cellulaire et d'Amélioration des Plantes UM CNRS-INRA 9938, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, F-69364 Lyon Cedex 07, France |
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Abstract: | The long-term viability of isolated embryo sacs was studied in maize. Fertilised embryo sacs were digested in order to remove most of the nucellus cells present on their surfaces and then transferred to culture. Experiments on 161 embryo sacs showed that isolation treatments using even minimal enzymatic digestion affected the further development of the embryo sacs. Few embryo sacs survived in culture and those produced only abnormal embryos; they produced no plants. We concluded that embryo sacs isolated through enzymatic digestion may offer limited prospects for long-term studies where normal embryogenic development is required. Alternative strategies are discussed for maize. |
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Keywords: | Embryo sac Zea mays Enzymatic isolation Zygotic embryogenesis Microinjection |
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