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Methodical investigations for selection of a histochemical routine-method of differentiating fibre types (author's transl)
Authors:I Fiedler  C Weber
Abstract:1. Transverse sections of two muscles of 20 pigs and 20 laboratory mice were studied methodically to ascertain a histochemical technique of differentiating fibre types suitable for routine-investigations. 2. The best results of four applied histochemical methods were obtained by the reaction for reduced disphosphopyridine nucleotide tetrazolium reductase (DPNH-TR). The comparison of the methods based on valuation-criterions as quality of reactions, expenditure of time and cost and requirements to the user. 3. The calculation of the variance-components showed for the evaluation of two samples (pigs) and one transverse section of muscle (mouse) per animal a good repeatability of the quantitative parameters of muscle fibre type composition.
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