Seasonal protein changes support rapid energy production in hibernator brainstem |
| |
Authors: | L Elaine Epperson James C Rose Rae L Russell Mrinalini P Nikrad Hannah V Carey Sandra L Martin |
| |
Institution: | (1) Department of Cell and Developmental Biology, University of Colorado School of Medicine, P.O. Box 6511, mail stop 8108, Aurora, CO 80045, USA;(2) Program in Molecular Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA;(3) Department of Comparative Biosciences, University of Wisconsin-Madison, 2015 Linden Dr., Madison, WI 53706, USA |
| |
Abstract: | During the torpor phase of mammalian hibernation when core body temperature is near 4°C, the autonomic system continues to
maintain respiration, blood pressure and heartbeat despite drastic reductions in brain activity. In addition, the hibernator’s
neuronal tissues enter into a protected state in which the potential for ischemia–reperfusion injury is markedly minimized.
Evolutionary adaptations for continued function and neuroprotection throughout cycles of torpor and euthermia in winter are
predicted to manifest themselves partly in changes in the brainstem proteome. Here, we compare the soluble brainstem protein
complement from six summer active ground squirrels and six in the early torpor (ET) phase of hibernation. Thirteen percent
of the ~1,500 quantifiable 2D gel spots alter significantly from summer to ET; the proteins identified in these differing
spots are known to play roles in energy homeostasis via the tricarboxylic acid cycle (8 proteins), cytoarchitecture and cell
motility (14 proteins), anabolic protein processes (13 proteins), redox control (11 proteins) and numerous other categories
including protein catabolism, oxidative phosphorylation, signal transduction, glycolysis, intracellular protein trafficking
and antiapoptotic function. These protein changes represent, at least in part, the molecular bases for restructuring of cells
in the brainstem, a shift away from glucose as the primary fuel source for brain in the winter, and the generation of a streamlined
mechanism capable of efficient and rapid energy production and utilization during the torpor and arousal cycles of hibernation. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|