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In vitro regeneration from leaf explants of Neoregelia cruenta (R. Graham) L.B. Smith,an endemic bromeliad from Eastern Brazil |
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| Authors: | Carneiro LA Araújo RFG Brito GJM Fonseca MHPB Costa A Crocomo OJ Mansur E |
| Institution: | 1.Laboratório de Micropropaga??o e Transforma??o de Plantas, Instituto de Biologia – DBCG, Universidade do Estado do Rio de Janeiro, Rua S?o Francisco Xavier 524 - PHLC sala 505, Rio de Janeiro, Brasil ;2.Centro de Biotecnologia Agrícola /ESALQ, USP, Brasil ;3.Depto. de Botanica, Museu Nacional, UFRJ, Brasil ; |
| Abstract: | An efficient plant regeneration system was developed for the induction of direct shoot formation from leaves derived from seedlings of Neoregelia cruenta, an endemic Bromeliaceae of Eastern Brazil. Shoot differentiation occurred directly from the leaf bases. In vitro responses were influenced by seedling age and growth regulator combinations. Highest regeneration rates were obtained from explants excised from 7-week-old seedlings cultured in the presence of 22 μM BA and 2.5 μM NAA. Shoot conversion to whole plants was most effective in shoots formed in response to 4.4 or 8.8 μM BA combined with 2.5 μM NAA. This revised version was published online in June 2006 with corrections to the Cover Date. |
| Keywords: | bromeliad endangered species micropropagation shoot formation |
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