Differences in the regulation of microtubule stability by the pro-rich region variants of microtubule-associated protein 4 |
| |
Authors: | Hasan Mohammad Rubayet Jin Mingyue Matsushima Kazuyuki Miyamoto Shigeaki Kotani Susumu Nakagawa Hiroyuki |
| |
Affiliation: | Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan. |
| |
Abstract: | We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 +/- 2.36 s, compared to 19.5 +/- 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites. |
| |
Keywords: | MAP, microtubule-associated protein SP, short pro-rich LP, long pro-rich FRAP, fluorescence recovery after photobleaching EGFP, enhanced green fluorescent protein EYFP, enhanced yellow fluorescent protein RB, reassembly buffer SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis ROI, region of interest |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|