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Differences in the regulation of microtubule stability by the pro-rich region variants of microtubule-associated protein 4 |
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| Authors: | Hasan Mohammad Rubayet Jin Mingyue Matsushima Kazuyuki Miyamoto Shigeaki Kotani Susumu Nakagawa Hiroyuki |
| Institution: | Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, Japan. |
| Abstract: | We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery after photobleaching analyses revealed that the interaction of MAP4-SP with the microtubules is very rapid, with a half-time of fluorescence recovery of 7 +/- 2.36 s, compared to 19.5 +/- 3.03 s in case of MAP4-LP. The dynamic interaction of MAP4-SP with microtubules in neural cells may contribute to the dynamic behaviors of extending neurites. |
| Keywords: | MAP microtubule-associated protein SP short pro-rich LP long pro-rich FRAP fluorescence recovery after photobleaching EGFP enhanced green fluorescent protein EYFP enhanced yellow fluorescent protein RB reassembly buffer SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis ROI region of interest |
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