Isolation and characterization of alkene-utilizing Xanthobacter spp. |
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| Authors: | C. G. van Ginkel J. A. M. de Bont |
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| Affiliation: | (1) Department of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, NL-6703 CT Wageningen, The Netherlands |
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| Abstract: | Yellow-pigmented bacteria showing typical characteristics of Xanthobacter spp. were isolated from enrichments with propene and 1-butene, using classical techniques. The generation time for growth on propene and 1-butene of these bacteria ranged from 5 to 7h. A NADH-dependent mono-oxygenase was identified in cell-free extract of Xanthobacter Py2. This mono-oxygenase was not influenced by potential inhibitors tested indicating that propene mono-oxygenase is different from other hydrocarbon mono-oxygenases described until now. Nitrogenase activity could be measured using the acetylene reduction assay with propene as energy source, because acetylene did not inhibit the mono-oxygenase activity. |
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| Keywords: | Propene 1-Butene Xanthobacter Mono-oxygenase Nitrogen fixation |
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