Culturability and survival of an extreme thermophile isolated from deep-sea hydrothermal vents |
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Authors: | J M González C Kato Koki Horikoshi |
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Institution: | (1) The Deepstar Group, Japan Marine Science and Technology Center (JAMSTEC), 2–15 Natsushima-cho, Yokosuka 237, Japan Tel. +81-468-67-5555; Fax +81-468-66-6364 e-mail: katoc@jamstec.go.jp, JP |
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Abstract: | The culturability of a strictly anaerobic, extremely thermophilic archaeon, Thermococcus peptonophilus (optimal growth temperature: 85° C), was studied during survival stages at various temperatures (98, 85, 70, and 4° C).
Total cell number (determined by DAPI staining), active cells (rhodamine-stained cells), and culturable cells (using most-probable-number)
were counted over time. The number of culturable cells decreased under each condition tested. The total number of cells significantly
decreased only at temperatures close to the maximum for growth (98° C); at this temperature, the cells spontaneously lysed.
Our results suggested that survival at 4° C in oxygenated waters might be a mechanism for the dispersion of extreme thermophiles
in the ocean. In addition, we proved the existence of T. peptonophilus cells in several physiological states: culturable cells, active non-culturable cells, inactive non-culturable cells, and
dead cells. Cell death was caused by cellular lysis.
Received: 5 February 1996 / Accepted: 16 April 1996 |
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Keywords: | Thermococcus peptonophilus Deep-sea Hydrothermal vents Survival Extreme thermophiles Culturability Active cells Mortality Dead cells |
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