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人巨细胞病毒对单纯疱疹病毒1型抗原表达的影响
引用本文:李向群,赵利淦,张美英,向近敏,梁浩麟,胡丽萍,陈保萍,方萍. 人巨细胞病毒对单纯疱疹病毒1型抗原表达的影响[J]. 中国微生态学杂志, 1993, 0(4)
作者姓名:李向群  赵利淦  张美英  向近敏  梁浩麟  胡丽萍  陈保萍  方萍
作者单位:湖北医科大学病毒所,湖北医科大学病毒所,湖北医科大学病毒所,湖北医科大学病毒所,湖北医科大学实验中心电镜室,湖北医科大学实验中心电镜室,湖北医科大学实验中心电镜室,湖北医科大学实验中心电镜室 武昌 430077,武昌 430077,武昌 430077,武昌 430077
摘    要:我们用免疫胶体金色埋前标记技术和免疫荧光技术研究了人胚肺细胞(HEL)内,人巨细胞病毒(HCMV-AD_(169))对单纯疱疹病毒1型(HSV-ⅠSM_(44))抗原表达的影响,旨在探讨在细胞这一微生境内,一病毒对另一病毒可能发生的影响。电镜下计数HSV-1组和HCMV HSV-1组特异性结合金颗粒数得HSV-1组为657个,HCMV HSV-1组的总数为283个。t检验P<0.01,差别非常显著。并且HSV-1组细胞的胞浆中的病毒颗粒,比HCMV HSV-1组明显多。荧光显微镜下:HSV-1组阳性细胞数为689个HCMV HSV-1组只有484个,经poisson分布u检验,P<0.01,差别非常显著。免疫荧光实验还表明:HSV-1组,抗血清在1:320时仍有荧光清晰的阳性细胞,而HCMV HSV-1组,抗血清在1:160时,却无荧光阳性细胞。细胞病变效应(CPE)动态观察显示:HSV-1组8小时即有细胞病变,24小时蔓延整个单层;而HCMV HSV-1组超感染14小时才有细胞病变。24小时约有75%细胞受累。结果表明HCMV对HSV-1的抗原表达有明显的抑制作用。对抑制作用的可能机理及其在分子生态学中的意义,进行了讨论。

关 键 词:HCMV和HSV-1双重感染  人胚肺细胞  HSV-1抗原表达

THE STUDY OF EFFECT OF HUMAN CYTOMEGALOVIRUS ON THE ANTIGEN EXPRESSION OF HERPES SIMPLEX VIRUS TYPE 1.
Li Xiang-qun. Zhao Li-gan. Zhang Mei-Yi. Xiang Jiang Jin-Ming viral research institute of Hubei Medical Vniversity. Wuhan. Liang Hao-Lin. Hu Li-Ping. Chen Bao-p ng. Fang-Ping. Electromicroscopy. THE STUDY OF EFFECT OF HUMAN CYTOMEGALOVIRUS ON THE ANTIGEN EXPRESSION OF HERPES SIMPLEX VIRUS TYPE 1.[J]. Chinese Journal of Microecology, 1993, 0(4)
Authors:Li Xiang-qun. Zhao Li-gan. Zhang Mei-Yi. Xiang Jiang Jin-Ming viral research institute of Hubei Medical Vniversity. Wuhan. Liang Hao-Lin. Hu Li-Ping. Chen Bao-p ng. Fang-Ping. Electromicroscopy
Affiliation:Li Xiang-qun. Zhao Li-gan. Zhang Mei-Yi. Xiang Jiang Jin-Ming viral research institute of Hubei Medical Vniversity. Wuhan. 430077 Liang Hao-Lin. Hu Li-Ping. Chen Bao-p ng. Fang-Ping. Electromicroscopy department of Experimental Cental of Hubei Medieal Vniversity. Wuhan. 430077
Abstract:Aiming at the molecular ecological aspects of the relationship of dual viral infection, we studied the interfering effects of HCMV on the antigen expression of HSV-1 in HEL cell by immuno-gold preembeded labelling electron microscopy and immunofluorescence assay. Total number of special conjugated gold particles and IF postive cell were recorded and compared. There were highly statisfically significant difference between the dual (HGMV HSV-1) and single (HSV-1) viral infection groups. The difference in CPE was also studied. The conclusion of this study is that HCMV showed a significant interfering effect on the antigen expression of HSV-1. The possible mechanism of interference and the meaning regarding the so-called molecular ecology was discussed.
Keywords:HCMV and HSV-1 dual infection. HEL. cell. Antigen expression of HSV-1.
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