MiRNA let-7g regulates skeletal myoblast motility via Pinch-2 |
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| Affiliation: | 1. CNRS FRE 3377, CEA Saclay, F-91191 Gif-sur-Yvette, France;2. Univ Paris-Sud, FRE 3377, F-91191 Gif-sur-Yvette, France;3. Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA;4. Centre de Recherche de Gif, Laboratoire d’Enzymologie et Biochimie Structurales, CNRS UPR3082, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France;5. Inserm U1016, Institut Cochin, INSERM U1016, CNRS 8104, Université Paris Descartes, F-75014 Paris, France |
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| Abstract: | Post-transcriptional regulation of gene expression by RNA-binding proteins and by small non-coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch-2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RNA-binding protein IMP-2/Igf2bp2. We now show that the expression of Pinch-2 is also regulated by the miRNA let-7g. Let-7g and IMP-2 repress Pinch-2 expression independently of each other. A knock-down of let-7g leads to an increase in Pinch-2 expression, and to a decrease of cell motility, which can be reversed by a simultaneous knock-down of Pinch-2. We conclude that let-7g controls the motility of mouse myoblasts in cell culture by post-transcriptionally regulating the expression of Pinch-2. |
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| Keywords: | Cell motility Cell adhesion microRNA RNA-binding protein Skeletal muscle PINCH-2 Let-7g |
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