Respiratory complex I from Escherichia coli does not transport Na+ in the absence of its NuoL subunit |
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Institution: | Instituto de Tecnologia Química e Biológica – António Xavier, Universidade Nova de Lisboa, Av. da Republica EAN, 2780-157 Oeiras, Portugal |
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Abstract: | We investigated H+ and Na+ transport by complex I from Escherichia coli devoid of the NuoL subunit, which is probably part of the ion translocating machinery. We observed that complex I devoid of the NuoL subunit still translocates H+, although to a smaller extension than the complete version of complex I, but does not transport Na+. Our results unequivocally reinforce the observation that E. coli complex I transports Na+ in the opposite direction to that of the H+ and show that NuoL subunit is involved in the translocation of both ions by complex I. |
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Keywords: | Respiratory chains Membrane proteins Hydrogenases Ion translocation |
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