MiR-20a regulates the PRKG1 gene by targeting its coding region in pulmonary arterial smooth muscle cells |
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| Institution: | 1. Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences, Shenzhen University, Shenzhen, Guangdong 518060, China;2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, Guangdong, China;3. Department of Chest Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518000, China;4. Department of Pediatrics, University of Illinois at Chicago, Chicago, IL 60612, USA |
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| Abstract: | Chronic hypoxia triggers pulmonary vascular remodeling, which is associated with de-differentiation of pulmonary artery smooth muscle cells (PASMC). Here, we show that miR-20a expression is up-regulated in response to hypoxia in both mouse and human PASMC. We also observed that miR-20a represses the protein kinase, cGMP-dependent, type I (PRKG1) gene and we identified two crucial miR-20a binding sites within the coding region of PRKG1. Functional studies showed that miR-20a promotes the proliferation and migration of human PASMC, whereas it inhibits their differentiation. In summary, we provided a possible mechanism by which hypoxia results in decreased PRKG1 expression and in the phenotypic switching of PASMC. |
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| Keywords: | MicroRNA cGMP-dependent protein kinase Coding region Pulmonary arterial smooth muscle cells Hypoxia |
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