Alt a 1 from Alternaria interacts with PR5 thaumatin-like proteins |
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Affiliation: | 1. Centre for Plant Biotechnology and Genomics (UPM-INIA), Pozuelo de Alarcon, Madrid, Spain;2. Institute of Applied Molecular Medicine (IMMA), CEU San Pablo University, Spain;3. Department of Biotechnology, ETSI Montes, Technical University Madrid, Spain |
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Abstract: | Alt a 1 is a protein found in Alternaria alternata spores related to virulence and pathogenicity and considered to be responsible for chronic asthma in children. We found that spores of Alternaria inoculated on the outer surface of kiwifruits did not develop hyphae. Nevertheless, the expression of Alt a 1 gene was upregulated, and the protein was detected in the pulp where it co-localized with kiwi PR5. Pull-down assays demonstrated experimentally that the two proteins interact in such a way that Alt a 1 inhibits the enzymatic activity of PR5. These results are relevant not only for plant defense, but also for human health as patients with chronic asthma could suffer from an allergic reaction when they eat fruit contaminated with Alternaria. |
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Keywords: | Alt a 1 PR5-thaumatin-like protein Kiwi-PR5 Competitive inhibitor Alternaria Kiwi |
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