Localization of human SAA gene(s) to chromosome 11 and detection of DNA polymorphisms |
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| Authors: | B Kluve-Beckerman S L Naylor A Marshall J C Gardner T B Shows M D Benson |
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| Institution: | 1. School of Agriculture, Food & Wine, University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia;2. Healthy Mothers, Babies and Children theme, South Australian Health and Medical Research Institute (SAHMRI), Adelaide, SA 5000, Australia;3. Neonatal Services, Women''s and Children''s Health Network, Adelaide, SA 5000, Australia;4. Women''s and Children''s Health Research Institute, North Adelaide, SA 5000, Australia;5. Discipline of Paediatrics, School of Medicine, University of Adelaide, Adelaide SA 5000, Australia;6. Nutrition and Dietetics, Flinders University, Adelaide, SA 5001, Australia |
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| Abstract: | A human serum amyloid A (SAA) cDNA was used as a probe in chromosome mapping studies to detect human SAA gene sequences in DNA isolated from human/mouse somatic cell hybrids. Southern analysis of DNA from 20 hybrid cell lines, including some with translocations of human chromosomes, placed the SAA gene(s) in the p11----pter region of chromosome 11. Screening of human DNA from unrelated individuals by Southern analysis using the SAA cDNA probe revealed restriction fragment polymorphisms for HindIII and PstI. An analysis of the segregation of these polymorphisms with other markers on the short arm of chromosome 11 should more precisely map the SAA gene(s). |
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