Purification and partial characterization of glutathione S-transferases from three field populations of Panonychus citri (Acari: Tetranychidae) |
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Authors: | Jin-Zhi Niu Wei Dou Bao-Jun Wang Guo-Na Zhang Rui Zhang Yi Yin Jin-Jun Wang |
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Institution: | (1) Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400716, People’s Republic of China; |
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Abstract: | Glutathione S-transferases (GSTs) play central roles in phase II detoxification of both xenobiotics (drugs, insecticides, and herbicides)
and endogenous compounds in almost all living organisms. In this study, we successfully purified the GSTs from the citrus
red mite, Panonychus citri, by affinity chromatography on Glutathione Sepharose 4B and compared the biochemical characterizations of the purified GSTs
from three field populations beibei (BB), wanzhou (WZ), and zhongxian (ZX)]. SDS–PAGE revealed that the molecular weight
of GSTs from three populations consisted of two subunits of 27.3 and 26.1 kDa. The specific activity of the purified GSTs
from the WZ and ZX populations was increased 1.5- and 3.8-fold, respectively, compared with the BB population. Accordingly,
the pyridaben susceptibility of WZ and ZX populations was less compared with BB population. Kinetic analyses showed that the
WZ and ZX populations had higher substrate specificity compared with the BB population based on the values of k
cat and k
cat
/K
m to both reduced glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). The in vitro inhibition studies of GSTs indicated
that the I
50 values of pyridaben from WZ and ZX populations of P. citri expressed 1.6- and 4.4-fold decreases, respectively, compared to the I
50 value of pyridaben from the BB population. In conclusion, all evidence suggested that the purified GSTs may partially contribute
to the susceptibility of acaricide pyridaben in field populations of P. citri. |
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Keywords: | |
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