Simultaneous quantification of lopinavir and ritonavir in human plasma by high performance liquid chromatography coupled with UV detection |
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Authors: | HuiJuan Kou Min Ye Qiang Fu Yang Han XiaoLi Du Jing Xie Zhu Zhu TaiSheng Li |
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Institution: | 1.Department of Infectious Diseases, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences-Peking Union Medical College,Beijing,China;2.Department of Pharmacy, Peking Union Medical College Hospital,Chinese Academy of Medical Sciences-Peking Union Medical College,Beijing,China |
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Abstract: | High performance liquid chromatography was coupled with UV detection for simultaneous quantification of lopinavir (LPV) and
ritonavir (RTV) in human plasma. This assay was sensitive, accurate and simple, and only used 200 μL of plasma sample. Samples
were liquid-liquid extracted, and diazepam was used as an internal standard. The chromatographic separation was achieved on
a C18 reversed-phase analytic column with a mobile phase of acetonitrile-sodium dihydrogen phosphate buffer (10 mmol L−1, pH 4.80) (60:40, v/v). UV detection was conducted at 205 nm and the column oven was set at 40°C. Calibration curves were
constructed between 0.5–20 μg mL−1 for LPV and 0.05–5 μg mL−1 for RTV. The relative standard deviations were 2.16%–3.20% for LPV and 2.12%–2.60% for RTV for intra-day analysis, and 2.34%–4.04%
for LPV and 0.31%–4.94% for RTV for inter-day analysis. The accuracy was within 100%±10%. The mean extraction recoveries were
79.17%, 52.26% and 91.35% for RTV, LPV and diazepam, respectively. This method was successfully applied to human plasma samples
from patients orally administered a salvage regimen of lopinavir-ritonavir tablets. |
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Keywords: | |
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