Plant regeneration from stem cortex expiant and protoplast cultures of Brassica juncea (mustard) |
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Authors: | A.-C. Bonfils A. Sproule J. A. Webb W. A. Keller |
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Affiliation: | (1) Biology Department, Tory Bldg., Carleton University, K1S 5B6 Ottawa, Ont., Canada;(2) Plant Research Centre, Bldg. 21, Agriculture Canada, K1A OC6 Ottawa, Ont., Canada;(3) Present address: Plant Biotechnology Institute, National Research Council, S7N OW9 Saskatoon, Sask., Canada |
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Abstract: | Summary Plant regeneration from stem cortex explants of 13 genotypes of Brassica juncea was assessed. Regeneration was strongly affected by genotype, as up to 50.6 shoots were produced per 100 calli of the most responsive line (Blaze), whereas no shoots were obtained from less responsive lines (Zeml, Vniimk351). Blaze was chosen for B. juncea stem cortex protoplast isolation. After one week of culture, 11–14% of the cells had divided, and about 0.002% produced 1–2 mm colonies within 6 weeks. Up to 7% of these colonies gave rise to shoots upon transfer to plant regeneration medium.Abbreviations 2,4-D 2,4-dichlorophenoxyaceticacid - BAP 6-benzylaminopurine - FDA fluorescein diacetate - IAA indole acetic acid - IBA indole butyric acid - 2ip 2-isopentenyl-adenine - NAA 1-naphthaleneacetic acid - TD thidiazuron |
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Keywords: | Brassica juncea Expiants Protoplasts Regeneration |
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