Lambda DNA在爪蟾卵非细胞系统中参与核组装的研究

以ＬａｍｂｄａＤＮＡ为外源性ＤＮＡ，爪蟾卵非细胞系统中进行核组装。在组装的不同时期提取核内和核外的ＤＮＡ，电泳检测显示其迁移率与ＬａｍｂｄａＤＮＡ完全相同，并随组装时间的延长，其含量在核内和核外分别是上升和下降的趋势。ＤＮａｓｅＩ能够降解核外的ＤＮＡ而不能降解核内的ＤＮＡ，证实了核膜的完整性。限制性内切酶分析进一步证明参加组装的ＤＮＡ就是ＬａｍｂｄａＤＮＡ。关键词

INCORPORATION OF EXOGNOUS LAMBDA DNA INTO ASSEMBLED NUCLEI IN XENOPUS CELL-FREE SYSTEM

By adding exogenous Lambda DNA to a cell-free system prepared form Xenopus eggs, nuclei were assembled.the intra-nuclei and extra-nuclei DNA were isolated at different Lambda DNa;and the contentof intra-nuclei and extra-nuclei DNA were increasing and decreasing, respectively, during the course of assembly. The extra-DNA was sensitive to DNase I, but intra-DNA could not be digested due to the protection of nuclear membrane. The restriction pattern of intra-DNA was the same as that of Lambda DNA. We conclude that the exogenous Lambda DNA is incorporated into the assembled nuclei.