EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B |
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Authors: | Budhaditya Banerjee Cortney A. Kestner P. Todd Stukenberg |
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Affiliation: | Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 |
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Abstract: | The Aurora B kinase coordinates kinetochore–microtubule attachments with spindle checkpoint signaling on each mitotic chromosome. We find that EB1, a microtubule plus end–tracking protein, is required to enrich Aurora B at inner centromeres in a microtubule-dependent manner. This regulates phosphorylation of both kinetochore and chromatin substrates. EB1 regulates the histone phosphorylation marks (histone H2A phospho-Thr120 and histone H3 phospho-Thr3) that localize Aurora B. The chromosomal passenger complex containing Aurora B can be found on a subset of spindle microtubules that exist near prometaphase kinetochores, known as preformed K-fibers (kinetochore fibers). Our data suggest that EB1 enables the spindle microtubules to regulate the phosphorylation of kinetochores through recruitment of the Aurora B kinase. |
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