ES细胞（MESPU13）嵌合体小鼠的GPI分析

为了评判小鼠ＥＳ细胞系ＭＥＳＰＵ１３的分化潜能，我们对１９只嵌合小鼠的心、肝、脾、肺、肾、胰腺、生殖腺、肌肉和血液的ＧＰＩ（磷酸葡萄糖异构酶）进行了分析。在这些样品中，来源于ＥＳ细胞的Ａ型条带的检出情况和小鼠的毛色嵌合率成正比关系。当毛色嵌合率低于４０％时，除了少数小鼠的肾脏外，没有看到Ａ型的条带。当毛色嵌合率大于８５％时，几乎所有的器官组织都检测到Ａ型条带，显示了ＥＳ细胞在发育形成内、中、外胚层的细胞方面具有很高的分化潜能。另外，在毛色嵌合率大于８５％的其中的６只嵌合鼠的肌肉中，只观察到Ａ型的条带，表明这些肌肉只单独来源于ＥＳ细胞。

The Analysis of GPI in Chimeric Mice of ES Cells(MESPU 13)

To estimate the differential potentiality of ES(Embryonic Stem)cell line MESPU 13,the heart,liver,spleen,lung,kidney,pancreas,gonad,muscle and blood of 19 chimeric mice were analyzed for GPI(Glucose Phosphate Isomeras)marker,in these studies,type A band from the ES cells,appeared parallel to the coat color chimerism of the mice.When coat color chimerism is below 40%,type A band was not seen except in the kidney of a few mice.Type A band was detected in nearly all the organs and tissues,when coat color chimerism was over 85%,indicated the ES cell has a fairly high potential to differentiate into cells of endoderm,mesoderm and ectoderm.In addition,only type A band was observed in the muscles of 6 mice which coat color chimerism is over 85%,the results indicated that these muscles were differentiated only from ES cells.