The spheroplast lysis assay for yeast in microtiter plate format. |
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Authors: | R Ovalle M Spencer M Thiwanont P N Lipke |
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Affiliation: | Department of Biological Sciences and Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York, New York 10021, USA. |
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Abstract: | A yeast lysis assay in the microtiter plate format improved precision and throughput and led to an improved algorithm for estimating lag time. The assay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determined to be the major source of variation. Microtiter-based assays should be useful for screening for drug susceptibility and for analyzing mutant phenotypes. |
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