Biological Comparison of Two Genotypes of Helicoverpa armigera Single-Nucleocapsid Nucleopolyhedrovirus |
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Authors: | Zhong-Jian Guo Jun-Qing Ge Dun Wang Ya-Ming Shao Qi-Yi Tang Chuan-Xi Zhang |
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Institution: | (1) Institute of Insect Sciences, Zhejiang University, Kaixuan Road 268, Hangzhou, 310029, Zhejiang, China |
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Abstract: | Baculovirus isolates from the same host species often show a considerable degree of variation on phenotypes. The completely
sequenced genotypes C1 and G4 of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HaSNPV) were compared. Bioassay studies suggested that nearly double of HaSNPV
G4 virus was required compared with HaSNPV C1 to achieve a similar LD50, and at the LD90 level the insect-killing speed for HaSNPV C1 was quicker than that of HaSNPV G4. The budded virus (BV) production of HaSNPV
C1 was nearly two- to threefold higher at 24 and 48 h post-infection (p.i.) than that of HaSNPV G4. However, the kinetics
of polyhedral inclusion body (PIB) formation in HzAM1 cells was similar in both the genotypes, which implied that the insect-killing
speed was not influenced by PIB formation, but by the kinetics of BV production. The results suggested that the HaSNPV C1
isolate was a better choice than HaSNPV G4 virus for controlling H. armigera. |
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Keywords: | bioassays budded virus production Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus polyhedral inclusion body formation |
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