A reliable genetic technique for sex determination of giant panda (<Emphasis Type="Italic">Ailuropoda melanoleuca</Emphasis>) from non-invasively collected hair samples |
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| Authors: | Matthew E Durnin Per J Palsbøll Oliver A Ryder Dale R McCullough |
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| Institution: | (1) Department of Environmental Science, Policy and Management, University of California Berkeley, 137 Mulford Hall, Berkeley, CA 94720, USA;(2) Center for Reproduction of Endangered Species, Zoological Society of San Diego, PO Box 120551, San Diego, CA 92112, USA;(3) Ornithology and Mammalogy, California Academy of Sciences, 875 Howard St., San Francisco, CA 94103, USA |
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| Abstract: | Extractions from non-invasive hair samples usually yield low amounts of highly degraded DNA. Previously developed mammal molecular
sexing methods were not designed with such sub-optimal conditions in mind. We developed a simple and reliable PCR-based sexing
method aimed at degraded, low yield DNA extractions from the giant panda (Ailuropoda melanoleuca). Comparisons of this new primer set with others showed that the reliability of sex determination from low-yield, degraded
DNA extractions was improved if; amplification products were short (<170 bp); and the Y-chromosome amplification product was
shorter than the X-chromosome amplification product. The primers developed in this study appear useful for sex determination
in other bear species. |
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| Keywords: | ZFX ZFY Sex Ursidae Bear Panda |
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