| 菰黑粉菌的分离鉴定及其发酵液中植物激素的检测 |
| |
| 引用本文： | 尤文雨,邹克琴,俞晓平,刘倩,安欣欣,赵煦泓,叶子弘.菰黑粉菌的分离鉴定及其发酵液中植物激素的检测[J].菌物学报,2010,29(2):178-184. |
| |
| 作者姓名： | 尤文雨 邹克琴 俞晓平 刘倩 安欣欣 赵煦泓 叶子弘 |
| |
| 作者单位： | 中国计量学院生命科学学院 杭州 310018 |
| |
| 基金项目： | 浙江省自然科学基金（No. Y306107）；浙江省科技计划重点项目（No. 2004C22003） |
| |
| 摘 要： | 在显微镜下观察孢子形态，并观察单菌落的形态和菌株的微观形态，PCR扩增其ITS-5.8S rDNA序列，测序并在NCBI中进行同源比较，确定其种属。液体培养该菌株，通过高效液相色谱法检测发酵液中植物激素。结果表明：用菌落形态与孢子形态鉴定和分子生物学鉴定的方法，对茭白中分离的一个菌株鉴定为菰黑粉菌，且在其发酵液中检测到植物激素IAA、ABA和GA3，其中IAA含量为0.1306mg/L，ABA含量为0.01367mg/L。
|
| 关 键 词： | 菰黑粉菌，形态，分子鉴定，HPLC |
Isolation and identification of Ustilago esculenta and detection of plant hormones in the fermentation broth |
| |
| Authors: | YOU Wen-Yu ZOU Ke-Qin YU Xiao-Ping LIU Qian AN Xin-Xin ZHAO Xu-Hong YE Zi-Hong |
| |
| Institution: | College of Life Science, China Jiliang University, Hangzhou 310018, China |
| |
| Abstract: | The morphology of spores and colonies were observed and the ITS-5.8S rDNA squences of the strains were cloned, sequenced and analyzed by using BLAST. The strain was cultured in liquid culture and plant hormones were detected by high performance liquid chromatography (HPLC). The result showed that the isolated strain was Ustilago esculenta, and indole acetic acid (IAA), abscisic acid (ABA), and gibberellic acid3 (GA3) were detected in the fermentation broth, and the contents of IAA is 0.1306mg/L, ABA is 0.01367mg/L. |
| |
| Keywords: | Ustilago esculenta morphology molecular identification HPLC |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
| 点击此处可从《菌物学报》下载免费的PDF全文 |
