Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes |
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Authors: | Alan Musgrave Hilda Kuin Marjan Jongen Piet de Wildt Frank Schuring Hans Klerk Herman van den Ende |
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Institution: | (1) Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098 SM Amsterdam, The Netherlands |
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Abstract: | Alcohols induce mating-structure activation in Chlamydomonas eugametos gametes. From the effect of ethanol on the 32P-labelling of polyphosphoinositides, we conclude that the synthesis of these lipids is stimulated. Biologically inactive concentrations of ethanol (<6%) had no effect on synthesis, but 6–8% ethanol stimulated synthesis for upto 60 min. The 32P incorporated into polyphosphoinositides and phosphatidic acid during ethanol treatment was readily chased out when 1 mM unlabelled Na3PO4 was added. Using a binding assay for inositol 1,4,5-trisphosphate, we show that the production of this phospholipid constituent is dramatically increased after ethanol treatment. This effect, coupled to a rise in intracellular calcium concentration, could explain gamete activation. The significance of these results in explaining other ethanol-induced phenomena in algae is discussed.Abbreviations Ins(1,4,5)P3
inositol 1,4,5-trisphosphate
- PtdA
phosphatidic acid
- PtdIns
phosphatidylinositol
- PtdIns(4)P
phosphatidylinositol 4-phosphate
- PtdIns(4,5)P2
phosphatidylinositol 4,5-bisphosphate
To whom correspondence should be addressedWe thank Dr. P. van Haastert (Biochemistry, University of Groningen, The Netherlands) and his colleagues for introducing us to their Ins(1,4,5)P3 assay, and Ben ten Brink (Molecular Cell Biology, University of Amsterdam, The Netherlands) for information about contractile vacuoles. We also thank Bas Nagelkerken, Marcel van der Vaart, Pieter van der Schoor, Gyuri Fenyvesi and Susan Kenter for their help. |
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Keywords: | Chlamydomonas Gamete (activation) Ethanol Inositol 1 4 5-trisphosphate Phospholipid turnover Signalling |
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