Biodetoxification of coumaphos insecticide using immobilizedEscherichia coli expressing organophosphorus hydrolase enzyme on cell surface |
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| Authors: | Ayman H Mansee Wilfred Chen Ashok Mulchandani |
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| Institution: | (1) Department of Chemical and Environmental Engineering, University of California, 92521 Riverside, CA, USA;(2) Pesticide Chemistry Department, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt |
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| Abstract: | Recently, we reported an improved technology for the degradation of organophosphate nerve agents using whole cells of genetically
engineeredEscherichia coli that anchored and displayed the enzyme organophosphorus hydrolase on the cell surface. In this paper we report the immobilization
of these cells on highly porous sintered glass beads and the subsequent application of the immobilized cell in a continuous-flow
packed bed bioreactor for the biodetoxification of a widely used insecticide, coumaphos. |
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| Keywords: | OPH pesticides nerve agents bioreactor porous sintered glass beads |
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