| 大豆GmCOL8基因的克隆及表达分析 |
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| 引用本文： | 马锦花,张清哲,陈新建,傅永福.大豆GmCOL8基因的克隆及表达分析[J].植物生理学通讯,2010(1):17-23. |
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| 作者姓名： | 马锦花 张清哲 陈新建 傅永福 |
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| 作者单位： | 河南农业大学生命科学学院;中国农业科学院作物科学研究所; |
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| 基金项目： | 国家支撑计划(2007BAD59B02); 转基因专项(2008ZX08009-001、2008ZX08004-005、2008ZX08010-004); “863”项目(2006AA10Z107、2006AA10A111、2007AA10Z119); “973”项目(2004CB117206) |
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| 摘 要： | 从大豆品种‘垦农18’中克隆了一个CONSTANS—like基因，命名为GmCOL8。进化树分析表明它属于CONSTANS—like亚家族1的成员。mRNA表达分析显示，GmCOL8在短日下具有明显的生物钟节律性表达特性，其表达高峰出现在凌晨。GmCOL8主要在复叶中表达，表达高峰出现在开花时期。
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| 关 键 词： | 大豆 CONSTANS-like 生物钟节律 生物信息学 |
Cloning and Expression Analysis of GmCOL8 Gene in Soybean (Glycine max L.) |
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| MA Jin-Hua,ZHANG Qing-Zhe,CHEN Xin-Jian,FU Yong-Fu,College of Life Sciences,Henan Agricultural University,Zhengzhou ,China,National Key Facility for Crop Gene Resources , Genetic Improvement.Cloning and Expression Analysis of GmCOL8 Gene in Soybean (Glycine max L.)[J].Plant Physiology Communications,2010(1):17-23. |
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| Authors: | MA Jin-Hua ZHANG Qing-Zhe CHEN Xin-Jian FU Yong-Fu College of Life Sciences Henan Agricultural University Zhengzhou China National Key Facility for Crop Gene Resources Genetic Improvement |
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| Institution: | MA Jin-Hua1,ZHANG Qing-Zhe1,CHEN Xin-Jian1,FU Yong-Fu2,1College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China,2National Key Facility for Crop Gene Resources , Genetic Improvement,Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081 |
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| Abstract: | A CONSTANS-like gene, named GmCOL8, was cloned from soybean （Glycine max） cultivar ＇Kennong 18＇ in the study. The analysis of phylogenetic tree showed that GmCOL8 was a member of soybean CONSTANS-Iike subfamily group 1. The mRNA expression pattern revealed that GmCOL8 had rhythmicity of circadian clock under the condition of short day, and reached a peak at dawn. It expressed mainly in trifoliolates, and the maximum level appeared at the stage of flowering. |
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| Keywords: | Glycine max CONSTANS-like circadian clock bioinformatics |
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