Morphology and SSU rDNA-based phylogeny of two Euplotes species from China: E. wuhanensis sp. n. and E. muscicola Kahl, 1932 (Ciliophora,Euplotida)
Affiliation:
1. Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University; Key Laborotory of Exploration and Utilization of Aquatic Genetic Resources; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;2. Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;3. Institute of Evolution & Marine Biodiversity, Key Laboratory of Mariculture of the Education Ministry of China, Ocean University of China, Qingdao 266003, China;4. Zoology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;1. Department of Biology, University of Pisa, 56126 Pisa, Italy;2. Laboratory of Eukaryotic Microbiology and Animal Biology, University of Camerino, 62032 Camerino, MC, Italy;1. Department of Biology, Gangneung-Wonju National University, Gangneung, South Korea;2. Consulting Engineering Office for Ecology, Radetzkystrasse 10, 5020 Salzburg, Austria;1. The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China;2. University of Innsbruck, Research Institute for Limnology, Mondseestrasse 9, 5310 Mondsee, Austria;3. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, China;4. Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;5. Consulting Engineering Office for Ecology, Radetzkystrasse 10, 5020 Salzburg, Austria;1. Institute of Evolution and Marine Biodiversity and Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;2. School of Life Sciences, East China Normal University, Shanghai 200241, China;3. Marine College, Shandong University, Weihai 264209, China;4. Department of Life Sciences, Natural History Museum, London SW7 5BD, UK;1. Laboratory of Protozoology, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life Science, South China Normal University, Guangzhou 510631, China;2. The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an, China;3. Laboratory of Protozoology, Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China;4. Department of Life Sciences, Natural History Museum, Cromwell Rd., London SW7 5BD, UK
Abstract:
The living morphology, infraciliature and silverline system of two small Euplotes species, E. wuhanensis sp. n. and E. muscicola Kahl, 1932, isolated from Wuhan, central China, were investigated. Euplotes wuhanensis sp. n. is characterized by a combination of features including small size (40–50 × 25–30 μm), two conspicuously small and eight normal-sized frontoventral cirri, five transverse cirri in two groups, two marginal and two caudal cirri, seven dorsal kineties with about 12 dikinetids in the mid-dorsal row and a double-eurystomus type of dorsal silverline pattern. The Wuhan population of E. muscicola closely resembles previously described populations. The establishments of three subspecies of E. muscicola are not supported. The small subunit ribosomal RNA gene sequences were determined for both species. We propose that the two sequences under the name of E. muscicola (No. AJ305254, DQ917684 deposited in GenBank) are very likely from misidentified material. Phylogenetic analyses based on these data support the validity of both E. muscicola and E. wuhanensis as distinct species.
