| 外源性GM_3／GD_3对人肝癌培养细胞蛋白激酶活性的影响 |
| |
| 引用本文： | 何伟,刘银坤,张夏英,宋家.外源性GM_3／GD_3对人肝癌培养细胞蛋白激酶活性的影响[J].中国生物化学与分子生物学报,1995,11(6):689-692. |
| |
| 作者姓名： | 何伟 刘银坤 张夏英 宋家 |
| |
| 作者单位： | 上海医科大学基础医学院生物化学教研室，中科院上海生物工程研究中心华新公司 |
| |
| 摘 要： | ＧＭ_３／ＧＤ_３在体外对部分纯化的酪氨酸蛋白激酶（ＴＰＫ）和蛋白激酶Ｃ（ＰＫＣ）都有激活作用。ＧＭ_３处理细胞４ｄ后，完整细胞ＴＰＫ活性未出现大改变，而ＰＫＣ的活性则大幅度上升，为对照组的８８倍；同样条件下经ＧＤ_３处理后，ＴＰＫ的活性下降，为对照组的０．６６倍，而ＰＫＣ活性则上升，为对照组的４３倍。上述结果表明，ＧＭ_３／ＧＤ_３对不同蛋白激酶活性的影响互不相同，对同一蛋白激酶在不同条件下的影响也互不相同。
|
| 关 键 词： | 蛋白激酶 GM_3 GD_3 |
| 收稿时间： | 1995-12-20 |
Studies on Effects of Exogeneous GM_3/GD_3 on Activities of Protein Kinases of Human Hepatocarcinoma Cell Line |
| |
| He,Wei,Liu,Yin-Kun,Zhang,Xia-Ying,Song,Jia-Xi.Studies on Effects of Exogeneous GM_3/GD_3 on Activities of Protein Kinases of Human Hepatocarcinoma Cell Line[J].Chinese Journal of Biochemistry and Molecular Biology,1995,11(6):689-692. |
| |
| Authors: | He Wei Liu Yin-Kun Zhang Xia-Ying Song Jia-Xi |
| |
| Institution: | (Department of Biochemistry,School of Basic Medicoal Sciences,Shanghai Medical Universty,Shanghai 200032) |
| |
| Abstract: | Both GM_3 and GD_3 can activate partially purified PCK ad TPK in vitro. After four-day treatment by GM_3 the activity of TPK remained unchanged,but the activity of PKC was 88-fold higher than that of the control;after the same treatment with GD_3,the activity of TPK decreased one fold,while the activity of PKC increased 43 folds. The results mentioned above indicated that GM_3/GD_3 had different effects on different protein kinases and showed an opposite tendency toward the two enzvmes. |
| |
| Keywords: | GM_3 GD_3 PKC TPK |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生物化学与分子生物学报》浏览原始摘要信息 |
| 点击此处可从《中国生物化学与分子生物学报》下载免费的PDF全文 |
